教育背景

• 2012.08-2017.07     清华大学，生物学学，博士

• 2008.09-2012.07     山东大学（威海），药学，学士

工作经历

• 2021.10~至今：上海交通大学转化医学研究院，长聘教轨副教授

• 2017.09~2021.09：芝加哥大学生物化学与分子生物系，博士后

研究方向

课题组致力于“hard-to-express”蛋白的功能探索、作用机制解析及调控策略开发等，具体研究对象包括翻译后修饰蛋白、毒素蛋白、镜像蛋白，具体研究包括1）Hard-to-express蛋白的合成策略开发与应用；2）Hard-to-express蛋白的作用机制解析及药物调控策略开发。

科研项目

• 国自然基金委面上项目，22277073，用于解析p97机制的蛋白化学泛素化新策略，2023-2026, 54万元，主持，在研；

• 上海市科学技术委员会，启明星计划项目，II型降解子的泛素化机制及PROTAC分子开发，2022-07至2025-06，40万元，主持，在研。

• 国家自然科学基金委员会，优秀青年科学基金项目（海外），泛素蛋白质机器的功能机制与化学调控，2022-01至2025-12，100-300万元，主持，在研。

代表性论文

独立PI期间

• Qingyun Zheng#; Tian Wang#; Junxiong Mao#; Guochao Chu; Lujun Liang; Yangwode Jing; Chong Zuo; Yuanyuan Yu; Honggang Hu＊; Man Pan＊; A Bifunctional Molecule-Assisted Synthesis of Mimics of Ubiquitin Chain, E2 Enzyme Intermediate, and Deubiquitinase Intermediate. Nature Protocols, 2022, in press.

• Huasong Ai#, Zebin Tong#, Zhiheng Deng, Jiakun Tian, Liying Zhang, Maoshen Sun, Yunxiang Du, Ziyu Xu, Qiang Shi, Lujun Liang, Qingyun Zheng, Jia-Bin Li, Man Pan＊, Lei Liu＊. Chemical synthesis of E2-ubiquitin-nucleosome conjugates to study E3-mediated protein ubiquitination through mechanism-based chemical trapping strategy. Chem 2022, under review.

博士-博后期间

• Man Pan#＊; Qingyun Zheng#; Tian Wang#; Lujun Liang#; Chong Zuo; Ruichao Ding; Huasong Ai; Yuan Xie; Si Dong; Yuanyuan Yu＊; Lei Liu＊; Minglei Zhao＊; Structural Insights Into the Initiation and Elongation of Ubiquitination by Ubr1, Nature, 2021, 600, 334–338.

• Man Pan#＊; Yuanyuan Yu#; Huasong Ai; Qingyun Zheng; Yuan Xie; Lei Liu＊; Minglei Zhao＊; Mechanistic insight into substrate processing and allosteric inhibition of human p97, Nature Structural & Molecular Biology, 2021, 28, 614-625.

• Man Pan#; Qingyun Zheng#; Yuanyuan Yu#; Huasong Ai; Yuan Xie; Xin Zeng; Chu Wang; Lei Liu＊; Minglei Zhao＊; Seesaw conformations of Npl4 in the human p97 complex and the inhibitory mechanism of a disulfiram derivative, Nature Communications, 2021, 12(1): 121.

• Man Pan#; Qingyun Zheng#; Shan Ding#; Lujia Zhang#; Qian Qu; Tian Wang; Danning Hong; Yujing Ren; Lujun Liang; Chunlai Chen＊; Ziqing Mei＊; Lei Liu＊; Chemical Protein Synthesis Enabled Mechanistic Studies on the Molecular Recognition of K27-linked Ubiquitin Chains, Angewandte Chemie International Edition, 2019, 9(58): 2627-2631.

• Man Pan#; Shuai Gao#; Yong Zheng#; Xiaodan Tan; Huan Lan; Xianglong Tan; Demeng Sun; Lining Lu; Tian Wang; Qingyun Zheng; Yichao Huang; Jiawei Wang; Lei Liu＊; Quasi-Racemic X-ray Structures of K27-Linked Ubiquitin Chains Prepared by Total Chemical Synthesis, Journal of the American Chemical Society, 2016, 23(138): 7429-7435.

• Man Pan#; Shan Li#; Xiang Li; Feng Shao; Lei Liu＊; Honggang Hu＊; Synthesis of and specific antibody generation for glycopeptides with arginine N-GlcNAcylation, Angewandte Chemie International Edition, 2014, 53(52): 14517-21.

• Man Pan#; Qingyun Zheng#; Shuai Gao; Qian Qu; Yuanyuan Yu; Ming Wu; Huan Lan; Yulei Li; Sanling Liu; Jiabin Li; Demeng Sun; Lining Lu; Tian Wang; Wenhao Zhang; Jiawei Wang; Yiming Li; Hong-Gang Hu＊; Changlin Tian＊; Lei Liu＊; Chemical Synthesis of Structurally Defined Phosphorylated Ubiquitins Suggests Impaired Parkin Activation by Phosphorylated Ubiquitins with a Non-Phosphorylated Distal Unit, CCS Chemistry, 2019, 1: 476-489.

• Yuanyuan Yu#; Qingyun Zheng#; Satchal K. Erramilli#; Man Pan#＊; Seongjin Park; Yuan Xie; Jingxian Li; Jingyi Fei; Anthony A. Kossiakoff＊; Lei Liu＊; Minglei Zhao＊; K29-Linked Ubiquitin Signaling Regulates Proteotoxic Stress Response and Cell Cycle, Nature Chemical Biology, 2021, 17: 896–905.（共一和共同通讯）

• Guo-Chao Chu#; Man Pan#; Jiabin Li#; Sanling Liu; Chong Zuo; Ze-Bin Tong; Jing-Si Bai; Qingyue Gong; Huasong Ai; Jian Fan; Xianbin Meng; Yi-Chao Huang; Jing Shi; Haiteng Deng; Changlin Tian; Yi-Ming Li; Lei Liu; Cysteine-Aminoethylation-Assisted Chemical Ubiquitination of Recombinant Histones, Journal of the American Chemical Society, 2019, 141: 3654-3663.

• Qian Qu#; Man Pan#; Shuai Gao; Qing-Yun Zheng; Yuan-Yuan Yu; Jia-Can Su; Xiang Li; Hong-Gang Hu; A Highly Efficient Synthesis of Polyubiquitin Chains, Advanced Science, 2018, 5: 1800234.

• Qingyun Zheng#; Tian Wang#; Guochao Chu#; Chong Zuo; Rui Zhao; Xin Sui; Linzhi Ye; Yuanyuan Yu; Jingnan Chen; Xiangwei Wu; Wenhao Zhang; Haiteng Deng; Jing Shi; Man Pan＊; Yiming Li＊; Lei Liu＊; An E1-Catalyzed Chemoenzymatic Strategy to Isopeptide-N-Ethylated Deubiquitylase-Resistant Ubiquitin Probes, Angewandte Chemie International Edition, 2020, 59(32): 13496-13501.

• Shuai Gao#; Man Pan#; Yong Zheng#; Yichao Huang; Qingyun Zheng; Demeng Sun; Lining Lu; Xiaodan Tan; Xianglong Tan; Huan Lan; Jiaxing Wang; Tian Wang; Jiawei Wang＊; Lei Liu＊; Monomer/Oligomer Quasi-Racemic Protein Crystallography, Journal of the American Chemical Society, 2016, 43(138): 14497-14502.

• Man Pan#; Yao He#; Ming Wen; Fangming Wu; Demeng Sun; Sijian Li; Longhua Zhang; Yiming Li; Changlin Tian＊; One-pot hydrazide-based native chemical ligation for efficient chemical synthesis and structure determination of toxin Mambalgin-1, Chemical Communications, 2014, 50(44): 5839.

• Demeng Sun#; You Yu#; Xiaobin Xue#; Man Pan#; Ming Wen; Siyu Li; Qian Qu; Xiaorun Li; Longhua Zhang; Xueming Li; Lei Liu; Maojun Yang; Changlin Tian; Cryo-EM structure of the ASIC1a–mambalgin-1 complex reveals that the peptide toxin mambalgin-1 inhibits acid-sensing ion channels through an unusual allosteric effect, Cell Discovery, 2018, 4(1): 27.